Research Paper Volume 13, Issue 1 pp 89—103

Further assessment of Salvia haenkei as an innovative strategy to counteract skin photo-aging and restore the barrier integrity

Effect of Salvia haenkei extract on HaCaT cell migration - wound healing assay. (A) Representative images showing the scratch (wound) at t = 0-3-6-12-24 h after treatment with 0.01-0.1 μg/ml SH. Images were acquired by confocal microscopy LSM 800 Software ZEN 2.1 Blue edition, 20X magnification and the scratch rate during the time was analyzed with the dedicated software. Figure (B) shows kymograms of HaCaT cells migration; (C) quantification of cells migration speed expressed as ratio of SH treatment to control (μm/h). Data are representative of 3 different experiments. *pppvs control.

Figure 7. Effect of Salvia haenkei extract on HaCaT cell migration - wound healing assay. (A) Representative images showing the scratch (wound) at t = 0-3-6-12-24 h after treatment with 0.01-0.1 μg/ml SH. Images were acquired by confocal microscopy LSM 800 Software ZEN 2.1 Blue edition, 20X magnification and the scratch rate during the time was analyzed with the dedicated software. Figure (B) shows kymograms of HaCaT cells migration; (C) quantification of cells migration speed expressed as ratio of SH treatment to control (μm/h). Data are representative of 3 different experiments. *p<0.05, **p<0.01, ***p<0.001 treatment vs control.