Figure 4. Influence of Linarin in LPS-medicated activation of NF-κB pathway in human chondrocytes. (A, C, D) The protein expressions of IκBα in cytoplasm and p65 in nuclear in chondrocytes treated as above were detected by western blot. (B) The nuclei translocation of p65 was detected by the immunofluorescence combined with DAPI staining for nuclei (scale bar: 20 μm). The data in the figures represent the averages ± S.D. Significant differences among different groups are indicated as ##P < 0.01, vs. control group; **P < 0.01 vs. LPS alone treatment group, n=5.