Research Paper Volume 13, Issue 4 pp 5383—5402

CircMRE11A_013 binds to UBXN1 and integrates ATM activation enhancing lens epithelial cells senescence in age-related cataract

LECs senescence was raising under UVB-inducing oxidative damage in vitro. (A). The number of SA-β-gal-positive SRA01/04 cells exposed under UVB were increased than controls. Scale bar: 25μm. (B). When UVB induced cells aging, the expression level of MRE11A mRNA was down-regulation. *pC). The expression level of circMRE11A was up-regulation compared with controls. ***pD). CCK-8 showed that the cell viability in UVB group was decreased. *pE). Flow cytometry demonstrated that cell cycle was inhibited in G1/S in UVB group. (F). Western Blot showed that the expression of ATM or MRE11A proteins were decreased, while the expression level of ATM-S1981p was increasing in UVB group compared with controls. Meanwhile, the relative increase of p53 and p21 was in UVB group compared with controls. (G) Immunofluorescence showed that p53 and p21 were co-located in the nucleus of cells. *p

Figure 3. LECs senescence was raising under UVB-inducing oxidative damage in vitro. (A). The number of SA-β-gal-positive SRA01/04 cells exposed under UVB were increased than controls. Scale bar: 25μm. (B). When UVB induced cells aging, the expression level of MRE11A mRNA was down-regulation. *p<0.05. (C). The expression level of circMRE11A was up-regulation compared with controls. ***p<0.001. (D). CCK-8 showed that the cell viability in UVB group was decreased. *p<0.05. (E). Flow cytometry demonstrated that cell cycle was inhibited in G1/S in UVB group. (F). Western Blot showed that the expression of ATM or MRE11A proteins were decreased, while the expression level of ATM-S1981p was increasing in UVB group compared with controls. Meanwhile, the relative increase of p53 and p21 was in UVB group compared with controls. (G) Immunofluorescence showed that p53 and p21 were co-located in the nucleus of cells. *p<0.05.