Research Paper Volume 13, Issue 4 pp 5383—5402

CircMRE11A_013 binds to UBXN1 and integrates ATM activation enhancing lens epithelial cells senescence in age-related cataract

CircMRE11A might bind to UBXN1 but not AGO2. (A). The Biotin-probes using in RNA pull-down were to target the back-splicing junction region of the circMRE11A. This experiment also designed with sufficient Biotin-probe as negative control (NC). (B). The MS analysis confirmed that there was no AGO2 protein in 35 unique proteins of the circMRE11A-probe group compared with the NC-probe group. (C). Western blot showed the AGO2 was not enabled to precipitate by the Biotin-circMRE11A probes from precipitated complexes. (D). We selected the UBXN1 and RNF10 in 35 unique proteins of circMRE11A group. The precipitated complexes were further confirmed by Western blot, only UBXN1 was determined at the higher level in the circMRE11A-probe group compared with controls, but not RNF10. The results were consistency with MS analysis. Input was total proteins as an internal control.

Figure 6. CircMRE11A might bind to UBXN1 but not AGO2. (A). The Biotin-probes using in RNA pull-down were to target the back-splicing junction region of the circMRE11A. This experiment also designed with sufficient Biotin-probe as negative control (NC). (B). The MS analysis confirmed that there was no AGO2 protein in 35 unique proteins of the circMRE11A-probe group compared with the NC-probe group. (C). Western blot showed the AGO2 was not enabled to precipitate by the Biotin-circMRE11A probes from precipitated complexes. (D). We selected the UBXN1 and RNF10 in 35 unique proteins of circMRE11A group. The precipitated complexes were further confirmed by Western blot, only UBXN1 was determined at the higher level in the circMRE11A-probe group compared with controls, but not RNF10. The results were consistency with MS analysis. Input was total proteins as an internal control.