Research Paper Volume 13, Issue 4 pp 5383—5402

CircMRE11A_013 binds to UBXN1 and integrates ATM activation enhancing lens epithelial cells senescence in age-related cataract

In vivo, circMRE11A-AAV2 was constructed and injected intravitreally in ICR mouse. (A). After intravitreal injection at 4 weeks, GFP-fluorescent protein encoded by circMRE11A-AAV2 or NC-AAV2 were detected in the lens of the mice under the fluorescence microscope in the circMRE11A-AAV2 group or the NC-AAV2 group compared with the control group. The results showed that circMRE11A-AAV2 had passed through lens capsule tissue and expressed in the lens of mice. (B). After intravitreal injection at 8 weeks, the opacification of lens in the circMRE11A-AAV2 group was observed compared with the control group under the slit lamp microscope. (C). Taking out the eyeball of mice, and the lens was observed under light microscope. (D). The number of SA-β-gal-positive cells increased in circMRE11A-AAV2 group compared with control group.

Figure 8. In vivo, circMRE11A-AAV2 was constructed and injected intravitreally in ICR mouse. (A). After intravitreal injection at 4 weeks, GFP-fluorescent protein encoded by circMRE11A-AAV2 or NC-AAV2 were detected in the lens of the mice under the fluorescence microscope in the circMRE11A-AAV2 group or the NC-AAV2 group compared with the control group. The results showed that circMRE11A-AAV2 had passed through lens capsule tissue and expressed in the lens of mice. (B). After intravitreal injection at 8 weeks, the opacification of lens in the circMRE11A-AAV2 group was observed compared with the control group under the slit lamp microscope. (C). Taking out the eyeball of mice, and the lens was observed under light microscope. (D). The number of SA-β-gal-positive cells increased in circMRE11A-AAV2 group compared with control group.