Research Paper Volume 13, Issue 4 pp 5475—5484

LncRNA PCGEM1 contributes to malignant behaviors of glioma by regulating miR-539-5p/CDK6 axis

Effects of PCGEM1 knockdown on glioma cells. (A) PCGEM1 expression was knocked down using shRNA targeting PCGEM1. (B) CCK8 assay for proliferation assessment. (C) EdU staining for assessing proliferation. (D) Colony formation assay was performed to evaluate proliferation. (E, F) Transwell assay for migration and invasion. (G) Animal xenograft experiment assay was performed and tumor volumes were measured at indicated time points. *P

Figure 2. Effects of PCGEM1 knockdown on glioma cells. (A) PCGEM1 expression was knocked down using shRNA targeting PCGEM1. (B) CCK8 assay for proliferation assessment. (C) EdU staining for assessing proliferation. (D) Colony formation assay was performed to evaluate proliferation. (E, F) Transwell assay for migration and invasion. (G) Animal xenograft experiment assay was performed and tumor volumes were measured at indicated time points. *P<0.05.