Figure 5. Overexpression of CRT significantly increases cell migration and decreases chemosensitivity of GBC-SD and NOZ cells to GEM. (A) CRT overexpression was confirmed by western blotting. (B) Cell migratory ability was detected by wound-healing assay. (C) Cell migratory ability was detected by Transwell assay. scale bars =200um. (D) Cell Counting Kit-8 assay was performed to analyze the proliferation of GBC-SD and NOZ cells. (E) Western blot assay suggested that p-Akt expression was involved in the chemosensitivity of GBC.*P<0.05 vs. negative control. p-, phosphorylated; CRT, calreticulin; GBC, gallbladder cancer; GEM, gemcitabine.