Research Paper Volume 13, Issue 4 pp 5650—5673

Synergistic inflammatory signaling by cGAS may be involved in the development of atherosclerosis

cGAS was positively correlated with atherosclerosis. ApoE -/- mice were fed either a western-type diet or a chow diet for 16 weeks. (A) Immunostaining of cGAS (green) and the macrophage marker MOMA-2 (red), the smooth muscle cell marker α-SMA (red), the endothelial cell marker CD31 (red) and their co-localization in atherosclerotic plaques. (n = 6, Scale bar: 100 μm). (B) Histological analysis of the aortic root stained with hematoxylin and eosin. (C) Oil Red-O analysis of lesion area as a percentage of in total aortic area in ApoE -/- mice. (D) The protein expression of cGAS in aorta was detected by western blotting. * P P P P

Figure 2. cGAS was positively correlated with atherosclerosis. ApoE -/- mice were fed either a western-type diet or a chow diet for 16 weeks. (A) Immunostaining of cGAS (green) and the macrophage marker MOMA-2 (red), the smooth muscle cell marker α-SMA (red), the endothelial cell marker CD31 (red) and their co-localization in atherosclerotic plaques. (n = 6, Scale bar: 100 μm). (B) Histological analysis of the aortic root stained with hematoxylin and eosin. (C) Oil Red-O analysis of lesion area as a percentage of in total aortic area in ApoE -/- mice. (D) The protein expression of cGAS in aorta was detected by western blotting. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. Data are means ± SD.