Research Paper Volume 13, Issue 4 pp 5650—5673

Synergistic inflammatory signaling by cGAS may be involved in the development of atherosclerosis

cGAS inhibition reduced lipid deposition and foam cell formation in RAW264.7 macrophages. RAW264.7 cells were treated for 24 hours with oxLDL(100 μg/mL) and with or without RU.521(cGAS inhibitor 2 μg/mL). (A) Oil Red-O staining or (B) BODIPY staining were used to identify lipid deposition and foam cell formation. RAW264.7 macrophages were treated with RU.521 (2 μg/mL) for 24 h before analysis. (C) Cholesterol uptake was determined using DiI-oxLDL (Scale bar: 20 μm). (D) HDL-mediated cholesterol efflux was assayed using NBD 485/535 (Scale bar: 20 μm). *** P P

Figure 3. cGAS inhibition reduced lipid deposition and foam cell formation in RAW264.7 macrophages. RAW264.7 cells were treated for 24 hours with oxLDL(100 μg/mL) and with or without RU.521(cGAS inhibitor 2 μg/mL). (A) Oil Red-O staining or (B) BODIPY staining were used to identify lipid deposition and foam cell formation. RAW264.7 macrophages were treated with RU.521 (2 μg/mL) for 24 h before analysis. (C) Cholesterol uptake was determined using DiI-oxLDL (Scale bar: 20 μm). (D) HDL-mediated cholesterol efflux was assayed using NBD 485/535 (Scale bar: 20 μm). *** P < 0.001, **** P < 0.0001. Data are means ± SD.