Figure 3. cGAS inhibition reduced lipid deposition and foam cell formation in RAW264.7 macrophages. RAW264.7 cells were treated for 24 hours with oxLDL(100 μg/mL) and with or without RU.521(cGAS inhibitor 2 μg/mL). (A) Oil Red-O staining or (B) BODIPY staining were used to identify lipid deposition and foam cell formation. RAW264.7 macrophages were treated with RU.521 (2 μg/mL) for 24 h before analysis. (C) Cholesterol uptake was determined using DiI-oxLDL (Scale bar: 20 μm). (D) HDL-mediated cholesterol efflux was assayed using NBD 485/535 (Scale bar: 20 μm). *** P < 0.001, **** P < 0.0001. Data are means ± SD.