**Figure 4.** **Transcriptome profiling of RU.521-treated RAW264.7 macrophages.** (**A**) Pearson’s correlation analysis of samples. The color reflects the intensity of the correlation. The higher the correlation coefficient between samples, the closer the expression pattern. (**B**) PCA analysis of samples. Principal component 1 (PC1) and principal component 2 (PC2) are indicated on the X-axis and Y-axis, respectively. Cos2 stands for variables. The closer the two samples were to each other, the smaller the difference was between the two samples in terms of gene expression patterns. (**C**) Volcano plot of the DEGs. The X-axis represents the difference in log 2 conversion, the Y-axis represents the significant difference after log 10 conversion, the blue represents the down-regulated DEGs, the red represents the up-regulated DEGs, and the gray represents the non-DEGs. The DEGs were defined according to the criteria of log2FC ≥2 and *P*adj <0.005. (**D**) Bar graph showing DEGs. The X-axis represents the type, the Y-axis represents gene counts, the green represents the downregulated DEGs, the blue represents the upregulated DEGs, and the red is the non-DEGs. (**E**) Hierarchical clustering heatmap. Red and green represent high and low relative expression, respectively. Rows represent DEGs, and columns represent samples. DEGs, differentially-expressed genes; PCA, principal component analysis.