Research Paper Volume 13, Issue 5 pp 6634—6661

CRISPR/Cas9-mediated CysLT1R deletion reverses synaptic failure, amyloidosis and cognitive impairment in APP/PS1 mice

CysLT1R deficiency ameliorates cognitive decline in APP/PS1 mice. (A) The mean escape latency to the visible platform during day 1–2. (B) The mean escape latency to the hidden platform during day 3–5. (C) The percentage of time stayed in the target quadrant, and (D) numbers of platform crossings during day 6. (E) Representative swim paths of mice. In the Y-maze test, (F) the number of correct choices on days 1-2 and (G) the latency to enter the safe compartment on day 2. In NORT, (H) discrimination index shown by the time spent exploring the novel object relative to the total time spent exploring both novel and familiar objects. In open field test, (I) the total distance traveled was analyzed. All values are expressed as mean ± SEM, n = 8, #P##P###P

Figure 4. CysLT1R deficiency ameliorates cognitive decline in APP/PS1 mice. (A) The mean escape latency to the visible platform during day 1–2. (B) The mean escape latency to the hidden platform during day 3–5. (C) The percentage of time stayed in the target quadrant, and (D) numbers of platform crossings during day 6. (E) Representative swim paths of mice. In the Y-maze test, (F) the number of correct choices on days 1-2 and (G) the latency to enter the safe compartment on day 2. In NORT, (H) discrimination index shown by the time spent exploring the novel object relative to the total time spent exploring both novel and familiar objects. In open field test, (I) the total distance traveled was analyzed. All values are expressed as mean ± SEM, n = 8, #P<0.05, ##P<0.01, ###P<0.001 vs. APP/PS1 mice.