Research Paper Volume 13, Issue 5 pp 6634—6661

CRISPR/Cas9-mediated CysLT1R deletion reverses synaptic failure, amyloidosis and cognitive impairment in APP/PS1 mice

CysLT1R deficiency alleviates neuroinflammation in APP/PS1 mice hippocampus. (A) GFAP+ astrocytes in hippocampal sections from different groups were detected. Scale bar = 50 μm. (B) The percentage of GFAP+-area was quantified. (C) CD68+ microglia in hippocampal sections from different groups were detected. Scale bar = 50 μm. (D) The percentage of CD68+-area was quantified. (E) IL-6 and (F) TNF-α in mice hippocampus were assessed by ELISA. All values are expressed as mean ± SEM, n = 4-6, #P, ##P, ###P

Figure 5. CysLT1R deficiency alleviates neuroinflammation in APP/PS1 mice hippocampus. (A) GFAP+ astrocytes in hippocampal sections from different groups were detected. Scale bar = 50 μm. (B) The percentage of GFAP+-area was quantified. (C) CD68+ microglia in hippocampal sections from different groups were detected. Scale bar = 50 μm. (D) The percentage of CD68+-area was quantified. (E) IL-6 and (F) TNF-α in mice hippocampus were assessed by ELISA. All values are expressed as mean ± SEM, n = 4-6, #P<0.05, ##P<0.01, ###P<0.001 vs. APP/PS1 mice.