Figure 2. MIF expression was upregulated in the posterior joint capsule after PTJC. (A) Western blot analysis of MIF in the posterior joint capsule at day 0, 1, 3, 7, and 14 after PTJC induction. Endogenous β-actin was used as an internal control. (B) Immunostaining showed colocalization of MIF (red) with JFs in the posterior joint capsule at day 0, 3, and 7 after PTJC induction. Cell nuclei were stained with DAPI (blue). Vimentin was used as a fibroblast marker (green). Scale bars, 20 μm. Error bars represent standard deviation. *P <0.05 compared with the day 0 group.