Figure 3. MIF expression increased in TNF-α-induced primary JF inflammation model. (A) Immunofluorescence of purified primary JFs. Vimentin was used as a marker of JFs (green). Cell nuclei were stained with DAPI (blue). Scale bar, 100 μm. (B–E) Expression of MIF in JFs in response to 20 ng/mL TNF-α treatment for 24 h was determined via qRT-PCR (B), ELISA (C), and immunofluorescence (D), and western blot (E). Phalloidin was used to stain the cytoskeleton (red); cell nuclei were stained with DAPI (blue). Scale bar, 100 μm. Error bars represent standard deviation. *P <0.05 compared with the PBS group.