Research Paper Volume 13, Issue 4 pp 5858—5874

GABARAP suppresses EMT and breast cancer progression via the AKT/mTOR signaling pathway

GABARAP suppresses the malignant behavior of breast cancer cells. (A) Knockdown of GABARAP in T47D and UACC-812 cells; GABARAP expression was determined using Western blot. (B) Overexpression of GABARAP in MDA-MB-453 cells; GABARAP expression was determined using Western blot. (C) Cell proliferation in T47D-vector, T47D-shRNA, UACC-812-vector and UACC-812-shRNA cells was detected using CCK-8 assays. (D) Cell proliferation in MDA-MB-453-vector and MDA-MB-453-GABARAP cells was detected using CCK-8 assays. (E) Colony-forming efficiency was determined in T47D-vector, T47D-shRNA, UACC-812-vector and UACC-812-shRNA cells. (F) Colony-forming efficiency was determined in MDA-MB-453-vector and MDA-MB-453-GABARAP cells. (G) Invasive abilities of T47D-vector, T47D-shRNA, UACC-812-vector and UACC-812-shRNA cells were measured using Matrigel invasion assays. (H) Invasive abilities of MDA-MB-453-vector and MDA-MB-453-GABARAP cells were measured using Matrigel invasion assays. (I) Migration abilities of T47D-vector, T47D-shRNA, UACC-812-vector and UACC-812-shRNA cells were assessed using wound-healing migration assays. (J) Migration abilities of MDA-MB-453-vector and MDA-MB-453-GABARAP cells were assessed using wound-healing migration assays. Experiments were performed at least three times. The data are expressed as the means ± SEM. P values were calculated using Student’s t-test. (*P P P

Figure 2. GABARAP suppresses the malignant behavior of breast cancer cells. (A) Knockdown of GABARAP in T47D and UACC-812 cells; GABARAP expression was determined using Western blot. (B) Overexpression of GABARAP in MDA-MB-453 cells; GABARAP expression was determined using Western blot. (C) Cell proliferation in T47D-vector, T47D-shRNA, UACC-812-vector and UACC-812-shRNA cells was detected using CCK-8 assays. (D) Cell proliferation in MDA-MB-453-vector and MDA-MB-453-GABARAP cells was detected using CCK-8 assays. (E) Colony-forming efficiency was determined in T47D-vector, T47D-shRNA, UACC-812-vector and UACC-812-shRNA cells. (F) Colony-forming efficiency was determined in MDA-MB-453-vector and MDA-MB-453-GABARAP cells. (G) Invasive abilities of T47D-vector, T47D-shRNA, UACC-812-vector and UACC-812-shRNA cells were measured using Matrigel invasion assays. (H) Invasive abilities of MDA-MB-453-vector and MDA-MB-453-GABARAP cells were measured using Matrigel invasion assays. (I) Migration abilities of T47D-vector, T47D-shRNA, UACC-812-vector and UACC-812-shRNA cells were assessed using wound-healing migration assays. (J) Migration abilities of MDA-MB-453-vector and MDA-MB-453-GABARAP cells were assessed using wound-healing migration assays. Experiments were performed at least three times. The data are expressed as the means ± SEM. P values were calculated using Student’s t-test. (*P < 0.05; **P < 0.01; ***P < 0.001).