Research Paper Volume 13, Issue 3 pp 4663—4673

Circular RNA circEPSTI1 accelerates cervical cancer progression via miR-375/409-3P/515-5p-SLC7A11 axis

CircEPSTI1 is highly expressed in cervical cancer cells and silencing of circEPSTI1 inhibits cell proliferation. (A) CircEPSTI1 expression in 2 cervical cancer cell lines (CaSki, HeLa) and normal cell line (HcerEpic) examined by qRT-PCR. (B) Knockdown efficacy of two distinct si-circEPSTI1 evaluated by RT-qPCR. (C) CCK-8 assay in HeLa and CaSki cells transfected with circEPSTI1 siRNAs or control. (D) HeLa and CaSki cancer cells were co-transfected with si-control, si- circEPSTI1 mimics. BrdUrd incorporation was evaluated via IHC to assess cell growth after transfection with the si-circEPSTI1 or si-control at 48 hours. **, P

Figure 1. CircEPSTI1 is highly expressed in cervical cancer cells and silencing of circEPSTI1 inhibits cell proliferation. (A) CircEPSTI1 expression in 2 cervical cancer cell lines (CaSki, HeLa) and normal cell line (HcerEpic) examined by qRT-PCR. (B) Knockdown efficacy of two distinct si-circEPSTI1 evaluated by RT-qPCR. (C) CCK-8 assay in HeLa and CaSki cells transfected with circEPSTI1 siRNAs or control. (D) HeLa and CaSki cancer cells were co-transfected with si-control, si- circEPSTI1 mimics. BrdUrd incorporation was evaluated via IHC to assess cell growth after transfection with the si-circEPSTI1 or si-control at 48 hours. **, P < 0.01 compared to the negative control group.