Research Paper Volume 13, Issue 4 pp 5986—6009

Figure 3. Knockdown or overexpression of hsa_circ_0002874 regulated the expression of miR1273f, MDM2 and P53 in A549 cell line. (A) 3 hsa_circ_0002874 siRNAs specifically targeting the backsplice junction sequences at different binding sites in hsa_circ_0002874 were designed. (B) hsa_circ_0002874 was determined by qPCR method after transfection of siRNAs-ciR and pCD25-ciR. (C) miR1273f was determined by qPCR method after transfection of siRNAs-ciR and pCD25-ciR. (D) MDM2 mRNA level was determined by qPCR method after transfection of siRNAs-ciR and pCD25-ciR. (E) MDM2 and P53 expression were determined by western blot after transfection of siRNAs-ciR and pCD25-ciR. (F) The quantification of MDM2 protein was analyzed according to bands in Figure 3E. (G) The quantification of P53 protein was analyzed according to bands in Figure 3E. *p <0.05, **p <0.01, ***p <0.001, n=3 in each group.