Research Paper Volume 13, Issue 4 pp 6010—6024

Exosomal long non-coding RNA LINC00662 promotes non-small cell lung cancer progression by miR-320d/E2F1 axis

Exosomal LINC00662 promotes proliferation and inhibits apoptosis of NSCLC cells. (A) The characteristics of exosomes were analyzed by the transmission electron microscopy (TEM) in the HCC827 and A549 cells. (B) The expression of CD9 and CD63 was measured by the Western blot analysis in the exosome of the HCC827 and A549 cells. (C) The expression of LINC00662 was tested by qPCR in the HCC827 and A549 cells treated with RNase A (1 μg/mL) or co-treated with RNase A (1 μg/mL) and Triton X100 (0.1%). (D–J) The HCC827 and A549 cells were infected with the lentiviral plasmids carrying LINC00662 shRNA (shLINC00662) or corresponding control shRNA (shNC), or transfected with the LINC00662 overexpression vector or the corresponding control vector, and the exosomes were extracted and further treated the cells. (D) The efficiency of the LINC00662 depletion and the LINC00662 overexpression was validated by qPCR assays in the cells. (E, F) The cell viability was analyzed by the MTT assays in the cells. (G, H) The cell proliferation was measured by the colony formation assays in the cells. (I, J) The cell apoptosis was measure by flow cytometry analysis in the cells. Data are presented as mean ± SD. Statistic significant differences were indicated: * P P

Figure 2. Exosomal LINC00662 promotes proliferation and inhibits apoptosis of NSCLC cells. (A) The characteristics of exosomes were analyzed by the transmission electron microscopy (TEM) in the HCC827 and A549 cells. (B) The expression of CD9 and CD63 was measured by the Western blot analysis in the exosome of the HCC827 and A549 cells. (C) The expression of LINC00662 was tested by qPCR in the HCC827 and A549 cells treated with RNase A (1 μg/mL) or co-treated with RNase A (1 μg/mL) and Triton X100 (0.1%). (DJ) The HCC827 and A549 cells were infected with the lentiviral plasmids carrying LINC00662 shRNA (shLINC00662) or corresponding control shRNA (shNC), or transfected with the LINC00662 overexpression vector or the corresponding control vector, and the exosomes were extracted and further treated the cells. (D) The efficiency of the LINC00662 depletion and the LINC00662 overexpression was validated by qPCR assays in the cells. (E, F) The cell viability was analyzed by the MTT assays in the cells. (G, H) The cell proliferation was measured by the colony formation assays in the cells. (I, J) The cell apoptosis was measure by flow cytometry analysis in the cells. Data are presented as mean ± SD. Statistic significant differences were indicated: * P < 0.05, ** P < 0.01.