Research Paper Volume 13, Issue 4 pp 6010—6024

Exosomal long non-coding RNA LINC00662 promotes non-small cell lung cancer progression by miR-320d/E2F1 axis

Exosomal LINC00662 promotes invasion and migration of NSCLC cells. (A–F) The HCC827 and A549 cells were infected with the lentiviral plasmids carrying LINC00662 shRNA (shLINC00662) or corresponding control shRNA (shNC), or transfected with the LINC00662 overexpression vector or the corresponding control vector, and the exosomes were extracted and further treated the cells. (A, B) The cell migration and invasion were examined by transwell assays in the cells. (C–F) The migration and invasion were measured by wound healing assays in the cells. The wound healing proportion was shown. (G–J) The cell cycle was analyzed by flow cytometry analysis in the cells. Data are presented as mean ± SD. Statistic significant differences were indicated: * P P

Figure 3. Exosomal LINC00662 promotes invasion and migration of NSCLC cells. (AF) The HCC827 and A549 cells were infected with the lentiviral plasmids carrying LINC00662 shRNA (shLINC00662) or corresponding control shRNA (shNC), or transfected with the LINC00662 overexpression vector or the corresponding control vector, and the exosomes were extracted and further treated the cells. (A, B) The cell migration and invasion were examined by transwell assays in the cells. (CF) The migration and invasion were measured by wound healing assays in the cells. The wound healing proportion was shown. (GJ) The cell cycle was analyzed by flow cytometry analysis in the cells. Data are presented as mean ± SD. Statistic significant differences were indicated: * P < 0.05, ** P < 0.01.