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Research Paper Volume 13, Issue 4 pp 6010—6024
Exosomal long non-coding RNA LINC00662 promotes non-small cell lung cancer progression by miR-320d/E2F1 axis
Figure 4. LINC00662 serves as a miR-320d sponge in NSCLC cells. (A) Potential interaction between lncRNA LINC00662 and miR-320d was identified by the bioinformatic analysis using ENCORI (http://starbase.sysu.edu.cn/index.php). (B) The expression levels of miR-320d were tested by qPCR in the HCC827 and A549 cells treated with control mimic (miR-NC) or miR-320d mimics. (C, D) Luciferase activities of LINC00662 (LINC00662 WT) and LINC00662 with the miR-320d-binding site mutant (LINC00662 MUT) were determined by luciferase reporter gene assays in the HCC827 and A549 cells treated with control mimic (miR-NC) or miR-320d mimic. (E) The efficiency of the LINC00662 depletion and the LINC00662 overexpression was validated by qPCR assays in the cells. (F) The HCC827 and A549 cells were treated with the lentiviral plasmids carrying LINC00662 shRNA (shLINC00662) or corresponding control shRNA (shNC), or transfected with the LINC00662 overexpression vector or the corresponding control vector. The expression of miR-320d was tested by qPCR assays in the cells. Data are presented as mean ± SD. Statistic significant differences were indicated: * P < 0.05, ** P < 0.01.