Research Paper Volume 13, Issue 5 pp 6681—6701

Indoxyl sulfate caused behavioral abnormality and neurodegeneration in mice with unilateral nephrectomy

Indoxyl sulfate decreased parameters of neurotrophins. Unilateral nephrectomized mice were intraperitoneally injected with indoxyl sulfate (IS, 0 and 100 mg/kg) and the indoxyl sulfate-injected mice were orally given with AST-120 (0 and 400 mg/kg) for 7 weeks. The serum samples were collected and subjected to the measurement of BDNF (A), serotonin (B), and corticosterone (C) levels. Proteins were extracted from the isolated prefrontal cortical tissues and subjected to Western blot with the indicated antibodies. Representative blots (D) and the quantitative data (E) are shown. Nuclear proteins were extracted from the isolated prefrontal cortical tissues and subjected to EMSA for measurement of CREB DNA binding activity. Representative blots (F) and the quantitative data (G) are shown. (H) The prefrontal cortical tissues were isolated and subjected to the measurement of PKA activity. Total RNAs were extracted from the isolated prefrontal cortical tissues and subjected to quantitative RT-PCR for the measurement of REST (I) and SNAP-25 (J) mRNA level. *p

Figure 6. Indoxyl sulfate decreased parameters of neurotrophins. Unilateral nephrectomized mice were intraperitoneally injected with indoxyl sulfate (IS, 0 and 100 mg/kg) and the indoxyl sulfate-injected mice were orally given with AST-120 (0 and 400 mg/kg) for 7 weeks. The serum samples were collected and subjected to the measurement of BDNF (A), serotonin (B), and corticosterone (C) levels. Proteins were extracted from the isolated prefrontal cortical tissues and subjected to Western blot with the indicated antibodies. Representative blots (D) and the quantitative data (E) are shown. Nuclear proteins were extracted from the isolated prefrontal cortical tissues and subjected to EMSA for measurement of CREB DNA binding activity. Representative blots (F) and the quantitative data (G) are shown. (H) The prefrontal cortical tissues were isolated and subjected to the measurement of PKA activity. Total RNAs were extracted from the isolated prefrontal cortical tissues and subjected to quantitative RT-PCR for the measurement of REST (I) and SNAP-25 (J) mRNA level. *p < 0.05 vs. control group, and #p < 0.05 vs. indoxyl sulfate alone (IS) group, n = 8.