Research Paper Volume 13, Issue 5 pp 6765—6781

Growth hormone ameliorates the age-associated depletion of ovarian reserve and decline of oocyte quality via inhibiting the activation of Fos and Jun signaling

Effects of GH administration in vivo on the ovarian reserve. (A) Schematic illustration for the NS and GH-treated mice. (B) The GH levels in the peripheral blood was measured in GH (n = 6) and vehicle (n = 7) group. (C) The changes of body weight and ovary index after GH administration. NS, no significance. (D) Micrographs of NS-treated and GH-treated mouse ovaries. Scale bar, 1 mm. (E) Left: Estrous cycle in representative females. Right: Average numbers of cycles in 8 days in two groups. P, proestrus; E, estrus; M, metestrus; D, diestrus. (F) HE-stained of NS-treated and GH-treated mouse ovaries. Scale bar, 400 μm, 200 μm. (G) Follicle counts and the number of corpus luteum in NS-treated (n = 3) and GH-treated (n = 5) mice. Data are presented as mean ± SD. *P P

Figure 3. Effects of GH administration in vivo on the ovarian reserve. (A) Schematic illustration for the NS and GH-treated mice. (B) The GH levels in the peripheral blood was measured in GH (n = 6) and vehicle (n = 7) group. (C) The changes of body weight and ovary index after GH administration. NS, no significance. (D) Micrographs of NS-treated and GH-treated mouse ovaries. Scale bar, 1 mm. (E) Left: Estrous cycle in representative females. Right: Average numbers of cycles in 8 days in two groups. P, proestrus; E, estrus; M, metestrus; D, diestrus. (F) HE-stained of NS-treated and GH-treated mouse ovaries. Scale bar, 400 μm, 200 μm. (G) Follicle counts and the number of corpus luteum in NS-treated (n = 3) and GH-treated (n = 5) mice. Data are presented as mean ± SD. *P < 0.05, **P < 0.01.