Research Paper Volume 13, Issue 5 pp 6765—6781

Growth hormone ameliorates the age-associated depletion of ovarian reserve and decline of oocyte quality via inhibiting the activation of Fos and Jun signaling

Effects of GH treatment in vivo on the quality and meiotic progress of aged oocytes. (A) Left: Representative images of MII oocytes, morula and blastocysts from NS-treated and GH-treated mice. Black arrows point to fragmented MII oocytes. Scale bar, 200 μm, 100 μm. Right: Number of MII oocytes and percentage of 2-cell embryos and blastocysts in NS-treated and GH-treated mice. (B) After cultured in M2 medium, the rate of GVBD and Pb1 extrusion were recorded. (C) Left: The MII oocytes from NS-treated (n = 37) and GH-treated (n = 42) mice were stained with α-tubulin (green) and propidium iodide (PI) (red). Scale bar, 50 μm. Right: Quantification of NS-treated and GH-treated oocytes with abnormal spindle/chromosomes. Data are presented as mean ± SD. *P P P

Figure 4. Effects of GH treatment in vivo on the quality and meiotic progress of aged oocytes. (A) Left: Representative images of MII oocytes, morula and blastocysts from NS-treated and GH-treated mice. Black arrows point to fragmented MII oocytes. Scale bar, 200 μm, 100 μm. Right: Number of MII oocytes and percentage of 2-cell embryos and blastocysts in NS-treated and GH-treated mice. (B) After cultured in M2 medium, the rate of GVBD and Pb1 extrusion were recorded. (C) Left: The MII oocytes from NS-treated (n = 37) and GH-treated (n = 42) mice were stained with α-tubulin (green) and propidium iodide (PI) (red). Scale bar, 50 μm. Right: Quantification of NS-treated and GH-treated oocytes with abnormal spindle/chromosomes. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.