Figure 5. ScRNA-seq of NS-treated and GH-treated MII oocytes. (A) Left: Mapping rate of NS-treated oocytes (n = 4) and GH-treated (n = 4) oocytes. Right: The number of detected genes (FPKM > 1) in vehicle and GH group oocytes. NS, no significance. (B) Left: The gene expression heatmap showed the differentially expressed genes (DEGs) in these two groups. Right: Principal components analysis (PCA) of eight samples. (C) The volcano map showed the DEGs between NS-treated and GH-treated mice. (D) The top 30 KEGG pathways involved in the down-regulated genes. The red box encloses the apoptosis pathway. (E) The top 22 KEGG pathways involved in the up-regulated genes. The red box encloses the GnRH signaling, cAMP signaling, calcium signaling and Rap1 signaling pathway. (F) 2 DEGs were selected for QPCR validation (n ≥ 10). Data are presented as mean ± SD. *P < 0.05, **P < 0.01.