Figure 6. GH treatment decreased oocyte apoptosis and DNA damage. (A) Left: Immunostaining for Ki-67 in ovaries from the NS and GH groups. Brown represents positive staining. Scale bar, 400, 100, 50 μm. Right: The mean density levels of Ki-67 in ovarian. (B) The ovaries from NS-treated and GH-treated mice were stained with γH2AX (red) and DAPI (blue). Scale bar, 50 μm. (C) Left: The MII oocytes from NS-treated (n = 7) and GH-treated (n=7) mice were stained with γH2AX (red) and hoechst (blue). Scale bar, 50 μm. Right: Mean density levels of NS-treated and GH-treated oocytes. Data are presented as mean ± SD. *P < 0.05, **P < 0.01.