Research Paper Volume 13, Issue 5 pp 6765—6781
Growth hormone ameliorates the age-associated depletion of ovarian reserve and decline of oocyte quality via inhibiting the activation of Fos and Jun signaling
Figure 6. GH treatment decreased oocyte apoptosis and DNA damage. (A) Left: Immunostaining for Ki-67 in ovaries from the NS and GH groups. Brown represents positive staining. Scale bar, 400, 100, 50 μm. Right: The mean density levels of Ki-67 in ovarian. (B) The ovaries from NS-treated and GH-treated mice were stained with γH2AX (red) and DAPI (blue). Scale bar, 50 μm. (C) Left: The MII oocytes from NS-treated (n = 7) and GH-treated (n=7) mice were stained with γH2AX (red) and hoechst (blue). Scale bar, 50 μm. Right: Mean density levels of NS-treated and GH-treated oocytes. Data are presented as mean ± SD. *P < 0.05, **P < 0.01.