Research Paper Volume 13, Issue 4 pp 6025—6040

SIRT6 enhances telomerase activity to protect against DNA damage and senescence in hypertrophic ligamentum flavum cells from lumbar spinal stenosis patients

Detection of differentially expressed genes in hypertrophic ligamentum flavum tissue samples from lumbar spinal stenosis patients. (A) A heat map was used to display differentially expressed genes (DEGs) identified when comparing non-hypertrophic and hypertrophic ligamentum flavum (LFN and LFH, respectively) tissue samples. Up- and down-regulated genes are shown in red and blue, respectively. n = 3. (B) DEGs were arranged in Volcano plots. (C) Differential SIRT6 and telomere function-related gene expression was confirmed via qRT-PCR, with GAPDH being used as a normalization control. n = 30. *pp

Figure 1. Detection of differentially expressed genes in hypertrophic ligamentum flavum tissue samples from lumbar spinal stenosis patients. (A) A heat map was used to display differentially expressed genes (DEGs) identified when comparing non-hypertrophic and hypertrophic ligamentum flavum (LFN and LFH, respectively) tissue samples. Up- and down-regulated genes are shown in red and blue, respectively. n = 3. (B) DEGs were arranged in Volcano plots. (C) Differential SIRT6 and telomere function-related gene expression was confirmed via qRT-PCR, with GAPDH being used as a normalization control. n = 30. *p<0.05, **p<0.01 vs. LFN.