Figure 6. CFTR knockdown abolishes the effects of CDX2 on resisting hyperglycemia-induced RTECs injury. NRK-52E cells were, respectively, non-transfected (NG group or HG group), and transfected with CDX2-overexpressing / Vector1 (HG+OE-CDX2 group / HG+Vector1 group) or CFTR-knockdown / Vector2 plasmid (HG+Sh-CFTR group / HG+Vector2 group), or co-transfected CDX2-overexpressing + CFTR-knockdown plasmid (HG+OE-CDX2+Sh-CFTR group). (A–C) Immunoblot bands of CDX2 and CFTR, and quantitative data (B, C). (D–I) Immunoblot bands of activated β-catenin, Snail, E-cadherin, Vimentin, and Col-III; quantitative data are shown (E–I). Data are mean±SD from three experiments performed independently. n=3; *P<0.05 versus HG+Vector1 group; #P<0.05 versus HG+Vector2 group; &P<0.05 versus HG+OE-CDX2 group.