Research Paper Volume 13, Issue 5 pp 6804—6819

Transcription factor ELK1 accelerates aerobic glycolysis to enhance osteosarcoma chemoresistance through miR-134/PTBP1 signaling cascade

PTBP1 facilitates chemoresistance of osteosarcoma cells to DXR by enhancing aerobic glycolysis in vitro. U2OS/DXR cells were treated with siRNA targeting PTBP1 and their parental cells were treated with expression vector containing PTBP1 gene. (A) The consumption of glucose and lactic acid and the ratio of ATP production in U2OS/DXR cells and their parental U2OS cells. (B) The consumption of glucose and lactic acid and the ratio of ATP production in U2OS/DXR cells and their parental U2OS cells. (C) Protein levels of GLUT3, HK2, and PDK 1(normalized to GAPDH) in U2OS/DXR cells and their  parental U2OS cells. U2OS/DXR cells were treated with siRNA targeting PTBP1, expression vector containing PTBP1 gene and/or siRNA-mediated knockdown of GLUT3, respectively, which were treated with 16 ug/ml DXR. (D) Cell viability and IC50 in U2OS/DXR cells. Values obtained from three independent experiments in triplicate are analyzed by unpaired t test between two groups and by ANOVA followed by Tukey's test among three or more groups. *p

Figure 4. PTBP1 facilitates chemoresistance of osteosarcoma cells to DXR by enhancing aerobic glycolysis in vitro. U2OS/DXR cells were treated with siRNA targeting PTBP1 and their parental cells were treated with expression vector containing PTBP1 gene. (A) The consumption of glucose and lactic acid and the ratio of ATP production in U2OS/DXR cells and their parental U2OS cells. (B) The consumption of glucose and lactic acid and the ratio of ATP production in U2OS/DXR cells and their parental U2OS cells. (C) Protein levels of GLUT3, HK2, and PDK 1(normalized to GAPDH) in U2OS/DXR cells and their parental U2OS cells. U2OS/DXR cells were treated with siRNA targeting PTBP1, expression vector containing PTBP1 gene and/or siRNA-mediated knockdown of GLUT3, respectively, which were treated with 16 ug/ml DXR. (D) Cell viability and IC50 in U2OS/DXR cells. Values obtained from three independent experiments in triplicate are analyzed by unpaired t test between two groups and by ANOVA followed by Tukey's test among three or more groups. *p < 0.05 compared with parental U2OS cells, or U2OS/DXR cells treated with si-NC; # p < 0.05 compared with U2OS cells treated with oe-NC; & p < 0.05 compared with U2OS/DXR cells treated with oe-PTBP1 +si-NC.