Research Paper Volume 13, Issue 5 pp 6820—6831

Nicardipine sensitizes temozolomide by inhibiting autophagy and promoting cell apoptosis in glioma stem cells

Combining dosing of nicardipine and TMZ decreased cell viability and promoted apoptosis of GSCs. SU4 cell viability was assessed by CCK-8 assay under exposure to TMZ (400 μM), nicardipine (20 μM) or both for 48 h (A). SU4 cells were treated with TMZ (400 μM), nicardipine (20 μM) or both for 48 h, then were stained with PI and Annexin V-FITC for apoptosis analysis (B, C). SU5 cell viability was assessed by CCK-8 assay under treatment with TMZ (400 μM), nicardipine (20 μM) or both for 48 h (D). SU5 cells were treated with TMZ (400 μM), nicardipine (20 μM) or both for 48 h, then were stained with PI and Annexin V-FITC for apoptosis analysis (E, F). Western blot analysis of Bcl-2, Bax, mitochondrial Bax and COX IV in GSCs was performed after treatment with TMZ, nicardipine or both for 48 h (G–K). * p p p p

Figure 2. Combining dosing of nicardipine and TMZ decreased cell viability and promoted apoptosis of GSCs. SU4 cell viability was assessed by CCK-8 assay under exposure to TMZ (400 μM), nicardipine (20 μM) or both for 48 h (A). SU4 cells were treated with TMZ (400 μM), nicardipine (20 μM) or both for 48 h, then were stained with PI and Annexin V-FITC for apoptosis analysis (B, C). SU5 cell viability was assessed by CCK-8 assay under treatment with TMZ (400 μM), nicardipine (20 μM) or both for 48 h (D). SU5 cells were treated with TMZ (400 μM), nicardipine (20 μM) or both for 48 h, then were stained with PI and Annexin V-FITC for apoptosis analysis (E, F). Western blot analysis of Bcl-2, Bax, mitochondrial Bax and COX IV in GSCs was performed after treatment with TMZ, nicardipine or both for 48 h (GK). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.