Figure 5. Rapamycin promoted GSCs autophagy by activating mTOR. The protein levels of p62, LC3 II and mTOR in GSCs were measured by Western blotting after exposure to TMZ (400 μM) plus nicardipine (20 μM) for 48 h, or in GSCs pretreated with rapamycin (50 nM) for 12 h (A). Statistical analysis of p62, LC3 II and mTOR expression in SU4 and SU5 cells (B–G). *p < 0.05, **p<0.01.