Research Paper Volume 13, Issue 4 pp 6041—6054

Long non-coding RNA CERS6-AS1 facilitates the oncogenicity of pancreatic ductal adenocarcinoma by regulating the microRNA-15a-5p/FGFR1 axis

CERS6-AS1 regulates FGFR1 expression in PDAC cells by sponging miR-15a-5p. (A, B) CERS6-AS1-depleted PANC-1 and SW1990 cells were analyzed by RT-qPCR and western blotting to quantify FGFR1 mRNA and protein levels, respectively. (C, D) si-CERS6-AS1 was cotransfected with miR-15a-5p inhibitor or NC inhibitor into PANC-1 and SW1990 cells. After transfection, RT-qPCR and western blotting were used to determine the mRNA and protein levels of FGFR1. (E) The correlation between FGFR1 mRNA and CERS6-AS1 expression in PDAC tissues was determined by Pearson’s correlation coefficient analysis. **P

Figure 5. CERS6-AS1 regulates FGFR1 expression in PDAC cells by sponging miR-15a-5p. (A, B) CERS6-AS1-depleted PANC-1 and SW1990 cells were analyzed by RT-qPCR and western blotting to quantify FGFR1 mRNA and protein levels, respectively. (C, D) si-CERS6-AS1 was cotransfected with miR-15a-5p inhibitor or NC inhibitor into PANC-1 and SW1990 cells. After transfection, RT-qPCR and western blotting were used to determine the mRNA and protein levels of FGFR1. (E) The correlation between FGFR1 mRNA and CERS6-AS1 expression in PDAC tissues was determined by Pearson’s correlation coefficient analysis. **P < 0.01.