Figure 5. CERS6-AS1 regulates FGFR1 expression in PDAC cells by sponging miR-15a-5p. (A, B) CERS6-AS1-depleted PANC-1 and SW1990 cells were analyzed by RT-qPCR and western blotting to quantify FGFR1 mRNA and protein levels, respectively. (C, D) si-CERS6-AS1 was cotransfected with miR-15a-5p inhibitor or NC inhibitor into PANC-1 and SW1990 cells. After transfection, RT-qPCR and western blotting were used to determine the mRNA and protein levels of FGFR1. (E) The correlation between FGFR1 mRNA and CERS6-AS1 expression in PDAC tissues was determined by Pearson’s correlation coefficient analysis. **P < 0.01.