Figure 2. LncRNA Sirt1-AS expression and Sirt1 activation in different groups of SAMP-1 mice. (A) Relative mRNA expression of Sirt1. (B) Relative expression of lncRNA Sirt1-AS. (C–E) Relative mRNA expression of p16, p53 and p21. (F–I) Relative protein expression of Sirt1, p16, p53, and p21. (J) p53 acetylation was detected. Error bars represent SD. *, p< 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.