Figure 6. Sirt1 is upregulated by lncRNA Sirt1-AS overexpression. (A, B) Expression of Sirt1 mRNA and lncRNA Sirt1-AS. (C–E) Relative mRNA expression of p16, p53 and p21. (F–I) Protein levels of Sirt1, p16, p53 and p21. (J) p53 acetylation was detected. (K) HE staining of endothelia of SAMP-1 mice. (40*). Error bars represent SD. *, p< 0.05; ***, p < 0.001; ****, p < 0.0001.