Research Paper Volume 13, Issue 5 pp 6918—6935

LncRNA Sirt1-AS upregulates Sirt1 to attenuate aging related deep venous thrombosis

Effects of lncRNA Sirt1-AS overexpression on HUVECs senescence. (A, B) The proportion of SA-β-Gal positive cells. (C–E) The contents of IL-8, MCP-1, IL-1α, IL-6, MMP-3 and MMP-9 after the transfection of lncRNA Sirt1-AS and treatment of Sirt1 inhibitor. (F) mRNA expression of senescence associated genes Icam1 and Serpine1. (G, H) The expression of senescence-associated markers rH2, K9M-H3, and HP1. Error bars represent SD. *, p

Figure 7. Effects of lncRNA Sirt1-AS overexpression on HUVECs senescence. (A, B) The proportion of SA-β-Gal positive cells. (CE) The contents of IL-8, MCP-1, IL-1α, IL-6, MMP-3 and MMP-9 after the transfection of lncRNA Sirt1-AS and treatment of Sirt1 inhibitor. (F) mRNA expression of senescence associated genes Icam1 and Serpine1. (G, H) The expression of senescence-associated markers rH2, K9M-H3, and HP1. Error bars represent SD. *, p< 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.