Figure 9. LncRNA Sirt1-AS regulated DVT through FOXO3a ubiquitination and degradation (A–D) Protein expression of Sirt1, FOXO3a and its acetylation were detected. (E) Relative mRNA expression of FOXO3a. (F) Co-IP was performed to detect the interaction of Sirt1 and FOXO3a. (G) Western blot was used to detect the FOXO3a ubiquitination level. (H, I) Western blot was used to detect the protein expression of FOXO3a after pcDNA3.1-FOXO3a transfection. (J) HE staining of endothelia of SAMP-1 mice. (40*) (K, L) proportion of SA-β-Gal positive cells. Error bars represent SD. *, p< 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.