Figure 6. EGB761 could increase synapse density, improve degeneration of synapse structure and prevent downregulation of synaptic proteins after CCH. The hippocampi tissues were made into ultramicrotomed sections with negative staining. The synapse was observed (A) (Bar scale= 1μm) and synapse density per vision fiend was counted (B). The structure of synapse was observed (C) (Bar scale= 250nm) and PSD area (D), relative PSD density (E), active zone length (F), average number of vesicles docking on AZ (G), presynaptic vesicles diameter (H), and presynaptic vesicle density (I) (n=3 for 4 group). NR1, NR2, synaptophysin, PSD95, and β-actin of the brain homogenates were examined by proteins blot and assayed (J–N).