Research Paper Volume 13, Issue 5 pp 7067—7083

GIT1 protects traumatically injured spinal cord by prompting microvascular endothelial cells to clear myelin debris

The clearance of myelin debris is reduced in the GIT1 KO mice after SCI. (A) IF staining of myelin debris marker MBP (green) and MECs marker CD31 (red) at the injury core of the GIT1 WT and KO mice at 3 and 7 days after SCI. Nuclei were stained using DAPI (blue). Bar = 500 μm. (B) Quantification of CD31-postive MECs and myelin-containing MECs in the injury sites at day 3 and 7 post-SCI. (C) Oil Red O images at day 7 post-SCI. Bar = 2000 μm. (D) Quantification of the ORO stained positive areas in the GIT1 WT and KO groups. N = 6 animals per group. *p **p

Figure 3. The clearance of myelin debris is reduced in the GIT1 KO mice after SCI. (A) IF staining of myelin debris marker MBP (green) and MECs marker CD31 (red) at the injury core of the GIT1 WT and KO mice at 3 and 7 days after SCI. Nuclei were stained using DAPI (blue). Bar = 500 μm. (B) Quantification of CD31-postive MECs and myelin-containing MECs in the injury sites at day 3 and 7 post-SCI. (C) Oil Red O images at day 7 post-SCI. Bar = 2000 μm. (D) Quantification of the ORO stained positive areas in the GIT1 WT and KO groups. N = 6 animals per group. *p < 0.05, **p < 0.01.