Research Paper Volume 13, Issue 5 pp 7096—7119

Berberine-induced TFEB deacetylation by SIRT1 promotes autophagy in peritoneal macrophages

Berberine promotes the formation of SIRT1/TFEB complex. (A) Changes in the expression of SIRT1/TFEB complex in peritoneal macrophages at different incubation time points after treatment with 100 μmol/L berberine. Data was analyzed by one-way ANOVA with Tukey HSD post-hoc test (vs. Control group). (B) The effect of NAM on SIRT1/TFEB complex expression after treatment with 100 μmol/L berberine for 6 h. Data was analyzed by one-way ANOVA with Tukey HSD post-hoc test (vs. Control group). Analysis of variance and Student-Newman-Keuls post hoc tests were used to compare two group (vs. berberine group). (C) The effect of TSA on SIRT1/TFEB complex after treatment with 100 μmol/L berberine for 6h. Data was analyzed by one-way ANOVA with Tukey HSD post-hoc test (vs. Control group). Analysis of variance and Student-Newman-Keuls post hoc tests were used to compare two group (vs. berberine group). (D, E) The effects of NAM and TSA on TFEB translocation in peritoneal macrophages at 6 h after treatment with 100 μmol/L berberine. Data was analyzed by one-way ANOVA with Tukey HSD post-hoc test (vs. Control group). Analysis of variance and Student-Newman-Keuls post hoc tests were used to compare two group (vs. berberine group). All values are expressed as mean ± SD (error bars) of three independent experiments. n = 3; *p #p

Figure 6. Berberine promotes the formation of SIRT1/TFEB complex. (A) Changes in the expression of SIRT1/TFEB complex in peritoneal macrophages at different incubation time points after treatment with 100 μmol/L berberine. Data was analyzed by one-way ANOVA with Tukey HSD post-hoc test (vs. Control group). (B) The effect of NAM on SIRT1/TFEB complex expression after treatment with 100 μmol/L berberine for 6 h. Data was analyzed by one-way ANOVA with Tukey HSD post-hoc test (vs. Control group). Analysis of variance and Student-Newman-Keuls post hoc tests were used to compare two group (vs. berberine group). (C) The effect of TSA on SIRT1/TFEB complex after treatment with 100 μmol/L berberine for 6h. Data was analyzed by one-way ANOVA with Tukey HSD post-hoc test (vs. Control group). Analysis of variance and Student-Newman-Keuls post hoc tests were used to compare two group (vs. berberine group). (D, E) The effects of NAM and TSA on TFEB translocation in peritoneal macrophages at 6 h after treatment with 100 μmol/L berberine. Data was analyzed by one-way ANOVA with Tukey HSD post-hoc test (vs. Control group). Analysis of variance and Student-Newman-Keuls post hoc tests were used to compare two group (vs. berberine group). All values are expressed as mean ± SD (error bars) of three independent experiments. n = 3; *p < 0.05 versus control. #p < 0.05 versus berberine group.