**Figure 3.** **Flow cytometry analysis of MMU and NMR cells upon treatment with DNA damaging agents.** (**A**). NMR and mouse cells were stained with FITC-Annexin V (AnnV) and Propidium Iodide (PI). The gating strategy for double negative AnnexinV^{-}/PI^{-}(Q3), single positive, early apoptotic cells (AnnexinV^{+}/PI^{-}) (Q4), single positive (AnnexinV^{-}/PI^{+}) (Q1) and double positive AnnexinV^{+}/PI^{+} (Q2) is shown. (**B**–**G**). Cell death of MMU (**B**–**D**) and NMR (**E**–**G**) cells under the wide spectrum of cytotoxic conditions was evaluated by FACS analysis. The total number of cells was taken as 100%; blue color indicates the population of viable cells, double negative AnnexinV^{-}/PI^{-}(Q3); yellow color is used for single positive, early apoptotic cells (AnnexinV^{+}/PI^{-}) cells (Q4); red color is implemented for double positive AnnexinV^{+}/PI^{+} cells (Q2); purple color for single positive (AnnexinV^{-}/PI^{+}) cells (Q1). The representative experiment out of three independent ones is shown.