Figure 4. Cytotoxicity and cytokine secretion analyses on aPDL1-CART cells in vitro. (A) The cytotoxic activity of effectors was evaluated by flow cytometry based on distinct labeling of target cells (mCherry+) and aPDL1-CART cells (EGFP+) following co-culture with PDL1-CA46 cells. (B) Cytotoxic effects of control T cells (red) and aPDL1-CART cells (blue) against PDL1-CA46 cells at different E:T ratios. * P < 0.05. (C) ELISA measurements of IL-2 and IFN-γ secretion in control T cells (red) and aPDL1-CART cells (blue). * P < 0.05.