Research Paper Volume 13, Issue 5 pp 7300—7313

Maf1 suppression of ATF5-dependent mitochondrial unfolded protein response contributes to rapamycin-induced radio-sensitivity in lung cancer cell line A549

Maf1 knockdown mitigates rapamycin inhibition of mitochondrial function in response to IR in A549 cells. (A, B) Mitochondrial membrane potential was evaluated by JC-1 staining followed by flow cytometry. Representative results are shown in (A) and the quantifications of 2 biological replicates are shown in (B). (C, D) Intracellular ROS levels were detected by H2DCFCA probes, followed by flow cytometry. Representative results are shown in (C) and the quantifications of 2 biological replicates are shown in (D). For all bar graph, the error bars stand for Standard Deviation (SD) of the mean. Statistical significance was evaluated by 2-tailed, paired student’s t-test (ns, not significant, *, P

Figure 4. Maf1 knockdown mitigates rapamycin inhibition of mitochondrial function in response to IR in A549 cells. (A, B) Mitochondrial membrane potential was evaluated by JC-1 staining followed by flow cytometry. Representative results are shown in (A) and the quantifications of 2 biological replicates are shown in (B). (C, D) Intracellular ROS levels were detected by H2DCFCA probes, followed by flow cytometry. Representative results are shown in (C) and the quantifications of 2 biological replicates are shown in (D). For all bar graph, the error bars stand for Standard Deviation (SD) of the mean. Statistical significance was evaluated by 2-tailed, paired student’s t-test (ns, not significant, *, P<0.05).