Research Paper Volume 13, Issue 4 pp 6156—6170

Extracellular vesicles from anoxia preconditioned mesenchymal stem cells alleviate myocardial ischemia/reperfusion injury

Characterization of mouse ADSCs and ADSC-derived EVs. (A) Analysis of cell surface markers (CD29, CD45, and CD90) in isolated mouse ADSCs; CD34, CD105, and CD106 were used as negative controls. (B) The purity of neonatal mouse cardiomyocytes was estimated to be higher than 95% based on α-actinin staining results. (C) TEM characterization of EVs. (D) Western blot examination of EV markers. Calnexin was used as negative control. (E) NanoSight’s light scattering intensity-based size distribution of isolated EVs. (F) EV tracer (PKH67) assay demonstrating successful EV internalization by cardiomyocytes.

Figure 1. Characterization of mouse ADSCs and ADSC-derived EVs. (A) Analysis of cell surface markers (CD29, CD45, and CD90) in isolated mouse ADSCs; CD34, CD105, and CD106 were used as negative controls. (B) The purity of neonatal mouse cardiomyocytes was estimated to be higher than 95% based on α-actinin staining results. (C) TEM characterization of EVs. (D) Western blot examination of EV markers. Calnexin was used as negative control. (E) NanoSight’s light scattering intensity-based size distribution of isolated EVs. (F) EV tracer (PKH67) assay demonstrating successful EV internalization by cardiomyocytes.