Research Paper Volume 13, Issue 4 pp 4881—4894

MiR-141-3p promotes mitochondrial dysfunction in ovariectomy-induced sarcopenia via targeting Fkbp5 and Fibin

OVX impairs mitochondrial function. (A, B) ATP content (A) and citrate synthase activity (B) in quadriceps muscle (n=8 mice per group). (C) Mitochondrial DNA content evaluated by the ratio of a mitochondrial encoded gene (Cox5b) and a nuclear-encoded gene (18S). (D) Relative mRNA expression of genes involved in mitochondrial biogenesis. (E) Extracellular flux analysis in EDF fiber from Sham or OVX mice (n=6). OCR was measured before (basal) and after successive addition of oligomycin (O) to determine ATP-linked respiration, carbonyl cyanide p-(trifluoromethoxy) phenylhydrazone (FCCP, F) to examine the maximal respiration, rotenone along with antimycin (R/A) to assess non-mitochondrial respiration. (F) Average OCR values were compared between Sham and OVX mice. Data are means ± SEM. *PPG) The levels of OXPHOS proteins in mitochondrial fraction. (H) Western blot analysis for NCoR1 and PGC1α/β. (I, J) The mRNA (I) and protein (J) expressions of ERR and PPARδ.

Figure 2. OVX impairs mitochondrial function. (A, B) ATP content (A) and citrate synthase activity (B) in quadriceps muscle (n=8 mice per group). (C) Mitochondrial DNA content evaluated by the ratio of a mitochondrial encoded gene (Cox5b) and a nuclear-encoded gene (18S). (D) Relative mRNA expression of genes involved in mitochondrial biogenesis. (E) Extracellular flux analysis in EDF fiber from Sham or OVX mice (n=6). OCR was measured before (basal) and after successive addition of oligomycin (O) to determine ATP-linked respiration, carbonyl cyanide p-(trifluoromethoxy) phenylhydrazone (FCCP, F) to examine the maximal respiration, rotenone along with antimycin (R/A) to assess non-mitochondrial respiration. (F) Average OCR values were compared between Sham and OVX mice. Data are means ± SEM. *P<0.05, **P<0.01; unpaired two-tailed Student’s t-test. (G) The levels of OXPHOS proteins in mitochondrial fraction. (H) Western blot analysis for NCoR1 and PGC1α/β. (I, J) The mRNA (I) and protein (J) expressions of ERR and PPARδ.