Research Paper Volume 13, Issue 6 pp 8127—8145

Long noncoding RNA NKILA transferred by astrocyte-derived extracellular vesicles protects against neuronal injury by upregulating NLRX1 through binding to mir-195 in traumatic brain injury

NKILA competitively binds to miR-195. (A) the subcellular location of NKILA in neurons predicted by the lncATLAS website. (B) the subcellular localization of NKILA in neurons detected by FISH assay (× 400). (C) the potential binding sites between NKILA and miR-195 predicted by RNA22. (D) the binding relationship between NKILA and miR-195 measured by dual-luciferase reporter gene assay. * p E) the enrichment of miR-195 caused by NKILA detected by RNA pull-down. * p F) the binding of NKILA and miR-195 with AGO2 determined by RIP assay. * p t test was used for pairwise comparison in panel D and F, and one-way ANOVA followed by Tukey’s post-hoc test was used for comparison in panel E. The cell experiment was repeated three times.

Figure 3. NKILA competitively binds to miR-195. (A) the subcellular location of NKILA in neurons predicted by the lncATLAS website. (B) the subcellular localization of NKILA in neurons detected by FISH assay (× 400). (C) the potential binding sites between NKILA and miR-195 predicted by RNA22. (D) the binding relationship between NKILA and miR-195 measured by dual-luciferase reporter gene assay. * p < 0.05 compared with the treatment of vector. (E) the enrichment of miR-195 caused by NKILA detected by RNA pull-down. * p < 0.05 compared with the treatment of Bio-probe NC. (F) the binding of NKILA and miR-195 with AGO2 determined by RIP assay. * p < 0.05 compared with IgG. All data were measurement data and expressed as mean ± standard deviation. Unpaired t test was used for pairwise comparison in panel D and F, and one-way ANOVA followed by Tukey’s post-hoc test was used for comparison in panel E. The cell experiment was repeated three times.