Research Paper Volume 13, Issue 4 pp 4831—4849

An aging and p53 related marker: HOXA5 promoter methylation negatively correlates with mRNA and protein expression in old age

DNAm of the target CpG sites of DUSP3, HOXA5 and RYR1. Mean DNA methylation values of the differentially methylated CpG sites are demonstrated in young and old subjects. The associated genes were assigned to the target CpG site, respectively. Detection of mean DNA methylation was performed by microarray on bisulfite-converted DNA prepared from monocytes. Error bars denote SEM (n = 4, average age: 23.8 yrs and 81.4 yrs, respectively). P values are FDR adjusted.

Figure 2. DNAm of the target CpG sites of DUSP3, HOXA5 and RYR1. Mean DNA methylation values of the differentially methylated CpG sites are demonstrated in young and old subjects. The associated genes were assigned to the target CpG site, respectively. Detection of mean DNA methylation was performed by microarray on bisulfite-converted DNA prepared from monocytes. Error bars denote SEM (n = 4, average age: 23.8 yrs and 81.4 yrs, respectively). P values are FDR adjusted.