Research Paper Volume 13, Issue 4 pp 6182—6193

Dexmedetomidine reverses MTX-induced neurotoxicity and inflammation in hippocampal HT22 cell lines via NCOA4-mediated ferritinophagy

DEX ameliorated MTX-induced iron overload and ROS overproduction in HT22 cells. (A–E) Fluorescence microscope with ROS Probe analysis and representative results of ROS in HT22 cells with different treatments. (F) Quantitative results of ROS fluorescence intensity in HT22 cells with different treatments. n=3; #pG) Quantitative results of total iron content in HT22 cells with different treatments. n=3; #pH–L) Fluorescence microscope with Ca-AM probe analysis and representative results of iron content in HT22 cells with different treatments. (M) Quantitative results of Ca-AM fluorescence intensity in HT22 cells with different treatments. n=3; #p

Figure 2. DEX ameliorated MTX-induced iron overload and ROS overproduction in HT22 cells. (AE) Fluorescence microscope with ROS Probe analysis and representative results of ROS in HT22 cells with different treatments. (F) Quantitative results of ROS fluorescence intensity in HT22 cells with different treatments. n=3; #p<0.05, vs Control; *p<0.05, vs MTX group. (G) Quantitative results of total iron content in HT22 cells with different treatments. n=3; #p<0.05, vs Control; *p<0.05, vs MTX group. (HL) Fluorescence microscope with Ca-AM probe analysis and representative results of iron content in HT22 cells with different treatments. (M) Quantitative results of Ca-AM fluorescence intensity in HT22 cells with different treatments. n=3; #p<0.05, vs Control; *p<0.05, vs MTX group.