Figure 4. DEX alleviated MTX-induced cytotoxicity and inflammatory injury in HT22 cells via NCOA4 mediated ferritinophagy. (A) Representative bands of Western blot analysis for the expression of NCOA4 in HT22 cells treated with NCOA4-siRNA. (B) The cell viability of HT22 cells in MTX, DEX and NCOA4-siRNA group were measured by CCK-8 assay. (C) The activity of LDH in HT22 cells with MTX, DEX and NCOA4-siRNA were measured using LDH assay kit. (D) The levels of IL-1β and (E) TNF-α in HT22 cells with MTX, DEX and NCOA4-siRNA were measured using ELISA. (F) The representative results of apoptotic cells in control, MTX (G), MTX+50ng/mLDEX+NC-siRNA (H), and MTX+50ng/mLDEX+NCOA4-siRNA (J) group were tested by flow cytometry. (K) Flow cytometry analysis of HT22 cells in MTX, DEX and NCOA4-siRNA group. n=3; #p<0.05, vs Control; *p<0.05, vs MTX group; &p<0.05, vs MTX+DEX+NC-siRNA.