Research Paper Volume 13, Issue 4 pp 6182—6193

Dexmedetomidine reverses MTX-induced neurotoxicity and inflammation in hippocampal HT22 cell lines via NCOA4-mediated ferritinophagy

Impact of NCOA4-siRNA on the expression of ferritinophagy related protein in HT22 cells with different treatments. (A–D) Western blot assay and representative results of NCOA4, FTH1 and LC3II/I in HT22 cells with different treatments. n=3; #pE) HT22 cells were co-stained with NCOA4 and LC3II. In representative images, NCOA4 and LC3II are shown in red and green, respectively.

Figure 5. Impact of NCOA4-siRNA on the expression of ferritinophagy related protein in HT22 cells with different treatments. (AD) Western blot assay and representative results of NCOA4, FTH1 and LC3II/I in HT22 cells with different treatments. n=3; #p<0.05, vs Control; *p<0.05, vs MTX group; &p<0.05, vs MTX+DEX+NC-siRNA (E) HT22 cells were co-stained with NCOA4 and LC3II. In representative images, NCOA4 and LC3II are shown in red and green, respectively.