Research Paper Volume 13, Issue 4 pp 6182—6193

Dexmedetomidine reverses MTX-induced neurotoxicity and inflammation in hippocampal HT22 cell lines via NCOA4-mediated ferritinophagy

DEX alleviated MTX-induced iron overload and ROS overproduction in HT22 cells via NCOA4 mediated ferritinophagy. (A, C) Fluorescence microscope with Ca-AM probe analysis and quantitative results of iron fluorescence intensity in HT22 cells with different treatments. n=3; #pB, D) Fluorescence microscope with ROS probe analysis and quantitative results of ROS fluorescence intensity in HT22 cells with different treatments. n=3; #pE) Quantitative results of total iron content in HT22 cells with different treatments. n=3; #p

Figure 6. DEX alleviated MTX-induced iron overload and ROS overproduction in HT22 cells via NCOA4 mediated ferritinophagy. (A, C) Fluorescence microscope with Ca-AM probe analysis and quantitative results of iron fluorescence intensity in HT22 cells with different treatments. n=3; #p<0.05, vs Control; *p<0.05, vs MTX group; &p<0.05, vs MTX+DEX+NC-siRNA. (B, D) Fluorescence microscope with ROS probe analysis and quantitative results of ROS fluorescence intensity in HT22 cells with different treatments. n=3; #p<0.05, vs Control; *p<0.05, vs MTX group; &p<0.05, vs MTX+DEX+NC-siRNA. (E) Quantitative results of total iron content in HT22 cells with different treatments. n=3; #p<0.05, vs Control; *p<0.05, vs MTX group; &p<0.05, vs MTX+DEX+NC-siRNA.