Figure 2. Gut microbiota analysis in the OVX and the Sham group mice. (A) Wayne diagram analysis based on OTU for the OVX group and the Sham group. (B) Shannon diversity index between OVX group (n=12) and Sham group (n=12) (t-test, P>0.05). (C) Simpson diversity index between OVX group (n=12) and Sham group (n=12) (t-test, P>0.05). (D, E) β-diversity analysis including principal component analysis (PCA) and OTU based partial least squares discrimination analysis (PLS-DA) between OVX and Sham group. (F, G) Significant differences analysis between OVX group and Sham group determined by linear discriminant analysis Effect Size (LEfSe). Data are presented as mean ± SEM. *P < 0.05, **P < 0.01. OTU: Operational Taxonomic Units; PCA: principal component analysis; PLS-DA: partial least squares discrimination analysis; LEfSe: Linear discriminant analysis Effect Size analysis.