**Figure 2.** **Gut microbiota analysis in the OVX and the Sham group mice.** (**A**) Wayne diagram analysis based on OTU for the OVX group and the Sham group. (**B**) Shannon diversity index between OVX group (n=12) and Sham group (n=12) (t-test, *P*>0.05). (**C**) Simpson diversity index between OVX group (n=12) and Sham group (n=12) (t-test, *P*>0.05). (**D**, **E**) β-diversity analysis including principal component analysis (PCA) and OTU based partial least squares discrimination analysis (PLS-DA) between OVX and Sham group. (**F**, **G**) Significant differences analysis between OVX group and Sham group determined by linear discriminant analysis Effect Size (LEfSe). Data are presented as mean ± SEM. **P* < 0.05, ***P* < 0.01. OTU: Operational Taxonomic Units; PCA: principal component analysis; PLS-DA: partial least squares discrimination analysis; *LEfSe*: Linear discriminant analysis Effect Size analysis.